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nonspecific primary antibody reactions  (Jackson Immuno)


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    Structured Review

    Jackson Immuno nonspecific primary antibody reactions
    Nonspecific Primary Antibody Reactions, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 4980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonspecific+primary+antibody+reactions/Normal+Donkey+Serum/pmc04689838-44-31-44
    Average 96 stars, based on 4980 article reviews
    nonspecific primary antibody reactions - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Saline:

    Article Title: Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments after Lumbar Disc Herniation in a Rat Model
    Article Snippet: .. To enhance antibody penetration into tissues, DRG sections were immersed in 50% ethanol for 30 min and rinsed with phosphate buffered saline (PBS) for 5 min, three times, and to block nonspecific primary antibody reactions, samples were treated with 10% normal donkey serum (NDS; Jackson Immunoresearch, Westgrove, PA, USA). .. Tissue sections were incubated overnight in a mixture of the following primary antibodies; mouse anti-Ionized calcium-binding adapter molecule 1 (Iba1) (Wako, Osaka, Japan; 1:1,000), mouse anti-glial fibrillary acidic protein (GFAP) (BD Pharmingen, San Jose, CA, USA; 1:100), anti-transient receptor potential vanilloid type 1 (TRPV1) (Neuromics, Edina, MN, USA; 1:5,000) and anti-calcitonin gene related peptide (CGRP) (Enzo, Farmingdale, NY, USA; 1:200).

    Blocking Assay:

    Article Title: Changes in the Expressions of Iba1 and Calcitonin Gene-Related Peptide in Adjacent Lumbar Spinal Segments after Lumbar Disc Herniation in a Rat Model
    Article Snippet: .. To enhance antibody penetration into tissues, DRG sections were immersed in 50% ethanol for 30 min and rinsed with phosphate buffered saline (PBS) for 5 min, three times, and to block nonspecific primary antibody reactions, samples were treated with 10% normal donkey serum (NDS; Jackson Immunoresearch, Westgrove, PA, USA). .. Tissue sections were incubated overnight in a mixture of the following primary antibodies; mouse anti-Ionized calcium-binding adapter molecule 1 (Iba1) (Wako, Osaka, Japan; 1:1,000), mouse anti-glial fibrillary acidic protein (GFAP) (BD Pharmingen, San Jose, CA, USA; 1:100), anti-transient receptor potential vanilloid type 1 (TRPV1) (Neuromics, Edina, MN, USA; 1:5,000) and anti-calcitonin gene related peptide (CGRP) (Enzo, Farmingdale, NY, USA; 1:200).



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    96
    Jackson Immuno nonspecific primary antibody reactions
    Nonspecific Primary Antibody Reactions, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nonspecific+primary+antibody+reactions/Normal+Donkey+Serum/pmc04689838-44-31-44
    Average 96 stars, based on 1 article reviews
    nonspecific primary antibody reactions - by Bioz Stars, 2026-09
    96/100 stars
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